In Vitro Evaluation of Larval Age and Dose of Virus- Inoculum on Mortality and Npv-yield from Helicoverpa

نویسندگان

  • ARMIGERA HUBNER
  • Mahesh Kumar
  • Magdeswar Sharma
  • Pradeep Kumar Sharma
  • Sardar Vallabh Bhai Patel
چکیده

Four larval instars viz. II,III ,IV & V were fed on diet treated with four different concentrations i.e. 1x10, 1x10, 1x10, 1x10 POB/ml to evaluate highest mortality and maximum viral yield. Findings suggest that Ha-NPV should be applied to III instar larvae in vitro to achieve high larval mortality (78.33 %) and maximum viral yield (4.319 x10 POB/g of cadavar) at 1x10ml dose inoculum. Mortality percentage reduced with a corresponding reduction in virus dosages in all studied instars.

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تاریخ انتشار 2012